1 September 25, 2019
Articles and Statements
1. Magda D. Davitashvili, Lamara D. Zuroshvili, Gela S. Azikuri
A Study of Antagonistic Relations of Microorganisms to Some Phytopathogenic Bacteria
Russian Journal of Biological Research, 2019, 6(1): 3-7.
Number of views: 850 Download in PDF
2. Ishaya Yohanna Longdet, Mankilik M. Mary, Offikwu Onyowo Maria, Idoko Edward DavidRussian Journal of Biological Research, 2019, 6(1): 3-7.
Abstract:
The relation of tubercle (Rhizobium trifoli, Rhizobium arachis, Rhizobium meliloti, Rhizobium phaseoli, Rhizobium lupini, Rhizobium leguminosarum) and rhizosphere microorganisms of legumes to some phytopathogenic bacteria (Pseudomonas tumefaciens, Corynebacterium michiganense, Pectobacterium carotovora, Pectobacterium aroidea, Xanthomonas vesicatoria, Pectobacterium phytophtorum, Xanthomonas campestris, Pseudomonas syringae) has been studied. Tubercle bacteria were found to reveal strong antagonistic properties, whereas rhizosphere microorganisms – medium.
The relation of tubercle (Rhizobium trifoli, Rhizobium arachis, Rhizobium meliloti, Rhizobium phaseoli, Rhizobium lupini, Rhizobium leguminosarum) and rhizosphere microorganisms of legumes to some phytopathogenic bacteria (Pseudomonas tumefaciens, Corynebacterium michiganense, Pectobacterium carotovora, Pectobacterium aroidea, Xanthomonas vesicatoria, Pectobacterium phytophtorum, Xanthomonas campestris, Pseudomonas syringae) has been studied. Tubercle bacteria were found to reveal strong antagonistic properties, whereas rhizosphere microorganisms – medium.
Number of views: 850 Download in PDF
Evaluation of the Antiplasmodial Activity of Diethyl Ether Leaf Extract of Eucalyptus Camaldulensis in Experimental Mice
Russian Journal of Biological Research, 2019, 6(1): 8-15.
Number of views: 887 Download in PDF
3. G.M. Raimova, M.K. Musaeva, N.N. Khoshimov, N.L. Vypova, K.E. NasirovRussian Journal of Biological Research, 2019, 6(1): 8-15.
Abstract:
The main aim of this research was to study the in vivo antiplasmodial activity of diethyl ether fraction of Eucalyptus camaldulensis leaf on malaria infection in experimental albino mice. The dried leaves were pounded into its powdered form with the aid of mortar and pestle. The plant leaf was extracted with Diethyl ether, yielded 4.03 g extract and the extract was screened for antiplasmodial activity. Mice infected with Plasmodium berghei were administered intraperitoneally doses ranging from 100-300 mg/kg body weight/day of the extract for 10 consecutive days. The positive control group was treated with 25 mg/kg of chloroquine. The diethyl ether extracts of Eucalyptus camaldulensis leaf shows suppressive activities on Plasmodium berghei in the animals treated. The group treated with standard drug (chloroquine) and the normal control group (uninfected untreated) survived beyond the experimental period, although the clearance time was faster for the standard drug. Changes in behavior and bodyweight were observed, this could be as a result of loss of appetite in the treated animal. Phytochemical screening revealed the presence of 6 phytochemicals including cardiac glycosides. The packed cell volume of the groups treated with diethyl ether extract of Eucalyptus camaldulensis decreased significantly as compared with both the positive and negative control groups while there is a slight increase in the normal control groups. From the result obtained, we can conclude that the diethyl ether leaf extract of Eucalyptus camaldulensis at 100 mg/kg, 200 mg/kg and 300 mg/kg body weight of mice reduced parasitemia level. So diethyl ether fraction of Eucalyptus camaldulensis was effective in the therapeutic management of malaria parasite.
The main aim of this research was to study the in vivo antiplasmodial activity of diethyl ether fraction of Eucalyptus camaldulensis leaf on malaria infection in experimental albino mice. The dried leaves were pounded into its powdered form with the aid of mortar and pestle. The plant leaf was extracted with Diethyl ether, yielded 4.03 g extract and the extract was screened for antiplasmodial activity. Mice infected with Plasmodium berghei were administered intraperitoneally doses ranging from 100-300 mg/kg body weight/day of the extract for 10 consecutive days. The positive control group was treated with 25 mg/kg of chloroquine. The diethyl ether extracts of Eucalyptus camaldulensis leaf shows suppressive activities on Plasmodium berghei in the animals treated. The group treated with standard drug (chloroquine) and the normal control group (uninfected untreated) survived beyond the experimental period, although the clearance time was faster for the standard drug. Changes in behavior and bodyweight were observed, this could be as a result of loss of appetite in the treated animal. Phytochemical screening revealed the presence of 6 phytochemicals including cardiac glycosides. The packed cell volume of the groups treated with diethyl ether extract of Eucalyptus camaldulensis decreased significantly as compared with both the positive and negative control groups while there is a slight increase in the normal control groups. From the result obtained, we can conclude that the diethyl ether leaf extract of Eucalyptus camaldulensis at 100 mg/kg, 200 mg/kg and 300 mg/kg body weight of mice reduced parasitemia level. So diethyl ether fraction of Eucalyptus camaldulensis was effective in the therapeutic management of malaria parasite.
Number of views: 887 Download in PDF
Selection and Study of Influence of Preparation “CMC” on the Process of Cooking of Blood
Russian Journal of Biological Research, 2019, 6(1): 16-21.
Number of views: 892 Download in PDF
4. Ishaya Yohanna Longdet, Deborah Haruna YakubuRussian Journal of Biological Research, 2019, 6(1): 16-21.
Abstract:
A study was carried out to identify a new structural fragment of coumarins from moldy clover (CMC) and to study the anticoagulant and toxicological characteristics. The quantitative and qualitative composition of the coumarin mixture was studied using spectrophotometry, fluorescence analysis and high-performance liquid chromatography. Coumarins and coumarinic acids were identified by absorption spectra and retention time in comparison with standard samples. The specificity of the method of direct spectrophotometry was assessed from the absorption spectra of clover grass extracts, the standard solution of coumarin solutions of model coumarin mixtures. The general orientation of the change in the clotting process under the action of drugs was judged by the records of a thromboelastogram performed on a thromboelastography (Tromb-2). The obtained fluorescence spectra as a function of the concentration of the extract showed a linear dependence of their intensity on the concentration, which made it possible to estimate the quantitative content of coumarins in CMC. The results show that the CMC really refers to the anticoagulants of indirect action, since the maximum CMC effect is manifested after 24 hours and is associated with a decrease in the content of procoagulants. By the effectiveness CMC is similar to the drug curantyl.
A study was carried out to identify a new structural fragment of coumarins from moldy clover (CMC) and to study the anticoagulant and toxicological characteristics. The quantitative and qualitative composition of the coumarin mixture was studied using spectrophotometry, fluorescence analysis and high-performance liquid chromatography. Coumarins and coumarinic acids were identified by absorption spectra and retention time in comparison with standard samples. The specificity of the method of direct spectrophotometry was assessed from the absorption spectra of clover grass extracts, the standard solution of coumarin solutions of model coumarin mixtures. The general orientation of the change in the clotting process under the action of drugs was judged by the records of a thromboelastogram performed on a thromboelastography (Tromb-2). The obtained fluorescence spectra as a function of the concentration of the extract showed a linear dependence of their intensity on the concentration, which made it possible to estimate the quantitative content of coumarins in CMC. The results show that the CMC really refers to the anticoagulants of indirect action, since the maximum CMC effect is manifested after 24 hours and is associated with a decrease in the content of procoagulants. By the effectiveness CMC is similar to the drug curantyl.
Number of views: 892 Download in PDF
Activity of Chloroform Extract of Eucalyptus camaldulensis Root against Plasmodium Berghei in vivo
Russian Journal of Biological Research, 2019, 6(1): 22-28.
Number of views: 916 Download in PDF
5. Russian Journal of Biological Research, 2019, 6(1): 22-28.
Abstract:
The work was designed to investigate the anti-malarial activity of chloroform root extract of Eucalyptus camaldulensis on Plasmodium berghei NK65 strain infection in vivo. Eighteen mice were intraperitoneal infected with chloroquine sensitive P. berghei strain and shared into 6 equal groups. Groups A, B, and C were treated, after infection, with 100, 200 and 300 mg extract/kg body weight of mouse respectively while group D was treated with 25 mg chloroquine/kg body weight. Group E mice were infected and administered only normal saline (negative control), and group F was neither infected nor treated. Phytochemical constituents of the plant extract were evaluated. The three concentrations of the extract resulted in reduced parasitemia, although the 200 mg/kg administered to group B had more effect than the 300mg/kg and 100 mg/kg administered to group C and A respectively. The highest activity was observed in the chloroquine group (positive control group). Also, at doses of 100 mg/kg, 200 mg/kg and 300 mg/kg, the extract produced increase in body weight and life span as compared to mice in the negative control group. At doses of 100mg/kg and 300mg/kg, the extract produced increase in PCV of the infected mice as compared to mice in the negative control group. Phytochemical screening showed that the leaf extract contains alkaloids, Balsam, Resin, cardiac glycosides and terpenes and steroids. The chloroform root extract of Eucalyptus camaldulensis presented a transient effect on Plasmodium infection in mice and so justifies the use of the plant as part of native desertion against malaria.
The work was designed to investigate the anti-malarial activity of chloroform root extract of Eucalyptus camaldulensis on Plasmodium berghei NK65 strain infection in vivo. Eighteen mice were intraperitoneal infected with chloroquine sensitive P. berghei strain and shared into 6 equal groups. Groups A, B, and C were treated, after infection, with 100, 200 and 300 mg extract/kg body weight of mouse respectively while group D was treated with 25 mg chloroquine/kg body weight. Group E mice were infected and administered only normal saline (negative control), and group F was neither infected nor treated. Phytochemical constituents of the plant extract were evaluated. The three concentrations of the extract resulted in reduced parasitemia, although the 200 mg/kg administered to group B had more effect than the 300mg/kg and 100 mg/kg administered to group C and A respectively. The highest activity was observed in the chloroquine group (positive control group). Also, at doses of 100 mg/kg, 200 mg/kg and 300 mg/kg, the extract produced increase in body weight and life span as compared to mice in the negative control group. At doses of 100mg/kg and 300mg/kg, the extract produced increase in PCV of the infected mice as compared to mice in the negative control group. Phytochemical screening showed that the leaf extract contains alkaloids, Balsam, Resin, cardiac glycosides and terpenes and steroids. The chloroform root extract of Eucalyptus camaldulensis presented a transient effect on Plasmodium infection in mice and so justifies the use of the plant as part of native desertion against malaria.
Number of views: 916 Download in PDF